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primary topic: Analysis of bacterial community composition of a spring water from the Western Ghats, India using culture dependent and molecular approaches.

Analysis of bacterial community composition of a spring water from the Western Ghats, India using culture dependent and molecular approaches.
type: AcademicArticle
type: Article
abstract: Cultivation based and culture independent molecular approaches were used to characterize the composition and structure of bacterial community from a natural warm spring in the Western Ghats, a biodiversity 'hotspot'. Dilution plating was done on three types of media with varying nutrient levels. Relatively nutritionally poor medium supported growth of highest number of bacteria (4.98 × 10(3) ml(-1)) compared to nutritionally rich media. On the basis of different morphological features on the plate, 62 aerobic and heterotrophic bacterial strains were isolated and their 16S rRNA genes were sequenced and analyzed. On the basis of sequence similarity these isolates were found to be distributed in 21 different genera belonging to Proteobacteria (58%) followed by Firmicutes (26%), Actinobacteria (13%) and Bacteroidetes (3%). Amplification of 16S rRNA gene of the community DNA using eubacterial primers, followed by cloning and sequencing revealed that predominant members of the habitat belong to the phylum Cyanobacteria (60%) followed by Proteobacteria (19.5%), Bacteroidetes (6.67%), Actinobacteria (4.4%) and Firmicutes (2.2%) and small ribosomal subunit of a plastid (of Chlorophyta, 2.2%).
date: 2011-01
volume: 62
issue: 1
is part of: Current microbiology
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
has document: Analysis of bacterial community composition of a spring water from the Western Ghats, India using culture dependent and molecular approaches. (PDF)
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has published: Analysis of bacterial community composition of a spring water from the Western Ghats, India using culture dependent and molecular approaches. (PDF)
has document: Analysis of bacterial community composition of a spring water from the Western Ghats, India using culture dependent and molecular approaches. (Indexer Terms)
subject: QR Microbiology

Analysis of bacterial community composition of a spring water from the Western Ghats, India using culture dependent and molecular approaches. (PDF)
type: Document

Current microbiology
type: Collection
issn: 14320991

T Chakrabarti
type: Person
given name: T
family name: Chakrabarti

Arunachalam Ruckmani
type: Person
given name: Arunachalam
family name: Ruckmani

Analysis of bacterial community composition of a spring water from the Western Ghats, India using culture dependent and molecular approaches. (Indexer Terms)
type: Document
is version of: Analysis of bacterial community composition of a spring water from the Western Ghats, India using culture dependent and molecular approaches. (PDF)
is volatile version of: Analysis of bacterial community composition of a spring water from the Western Ghats, India using culture dependent and molecular approaches. (PDF)
is index codes version of: Analysis of bacterial community composition of a spring water from the Western Ghats, India using culture dependent and molecular approaches. (PDF)

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primary topic: Analysis of bacterial community composition of a spring water from the Western Ghats, India using culture dependent and molecular approaches.

QR Microbiology
type: Concept