@article{open114,
          volume = {153},
          number = {1-2},
           month = {August},
          author = {G.P.S. Raghava and A Joshi and J N Agrewala},
            note = {Copyright of this article belongs to Elsevier Science.},
           title = {Calculation of antibody and antigen concentrations from ELISA data using a graphical method.},
       publisher = {Elsevier Science},
            year = {1992},
         journal = {Journal of immunological methods},
           pages = {263--4},
             url = {http://crdd.osdd.net/open/114/},
        abstract = {A graphical method for determining the concentration of either the antibody or the antigen from ELISA data is presented in the form of a GWBASIC program. In the program, ELISAEQ, optical densities (OD) obtained from a 96-well ELISA plate can be input either directly by interfacing a microplate reader to the computer or manually. The program uses standard sample data, and selects the semilogarithmic linear range. Over this range, a least-squares method is used to determine the concentrations of interest. In addition, a hyperbolic interpolation formula is derived over the entire range for estimating the antibody or antigen concentration of the unknown samples whose OD is beyond the linear range.}
}