%0 Journal Article
%@ 0022-1759
%A Raghava, G.P.S.
%A Joshi, A
%A Agrewala, J N
%D 1992
%F open:114
%I Elsevier Science
%J Journal of immunological methods
%N 1-2
%P 263-4
%T Calculation of antibody and antigen concentrations from ELISA data using a graphical method.
%U http://crdd.osdd.net/open/114/
%V 153
%X A graphical method for determining the concentration of either the antibody or the antigen from ELISA data is presented in the form of a GWBASIC program. In the program, ELISAEQ, optical densities (OD) obtained from a 96-well ELISA plate can be input either directly by interfacing a microplate reader to the computer or manually. The program uses standard sample data, and selects the semilogarithmic linear range. Over this range, a least-squares method is used to determine the concentrations of interest. In addition, a hyperbolic interpolation formula is derived over the entire range for estimating the antibody or antigen concentration of the unknown samples whose OD is beyond the linear range.
%Z Copyright of this article belongs to Elsevier Science.