TY  - JOUR
N1  - Copyright of this article belongs to Elsevier Science.
ID  - open114
UR  - http://www.sciencedirect.com/science/article/pii/002217599290330V
IS  - 1-2
A1  - Raghava, G.P.S.
A1  - Joshi, A
A1  - Agrewala, J N
Y1  - 1992/08/30/
N2  - A graphical method for determining the concentration of either the antibody or the antigen from ELISA data is presented in the form of a GWBASIC program. In the program, ELISAEQ, optical densities (OD) obtained from a 96-well ELISA plate can be input either directly by interfacing a microplate reader to the computer or manually. The program uses standard sample data, and selects the semilogarithmic linear range. Over this range, a least-squares method is used to determine the concentrations of interest. In addition, a hyperbolic interpolation formula is derived over the entire range for estimating the antibody or antigen concentration of the unknown samples whose OD is beyond the linear range.
PB  - Elsevier Science
JF  - Journal of immunological methods
VL  - 153
SN  - 0022-1759
TI  - Calculation of antibody and antigen concentrations from ELISA data using a graphical method.
SP  - 263
AV  - none
EP  - 4
ER  -