creators_name: Raghava, G.P.S.
creators_name: Joshi, A
creators_name: Agrewala, J N
type: article
datestamp: 2012-01-09 07:16:31
lastmod: 2014-03-28 10:57:27
metadata_visibility: show
title: Calculation of antibody and antigen concentrations from ELISA data using a graphical method.
ispublished: pub
subjects: QR180
full_text_status: none
note: Copyright of this article belongs to Elsevier Science.
abstract: A graphical method for determining the concentration of either the antibody or the antigen from ELISA data is presented in the form of a GWBASIC program. In the program, ELISAEQ, optical densities (OD) obtained from a 96-well ELISA plate can be input either directly by interfacing a microplate reader to the computer or manually. The program uses standard sample data, and selects the semilogarithmic linear range. Over this range, a least-squares method is used to determine the concentrations of interest. In addition, a hyperbolic interpolation formula is derived over the entire range for estimating the antibody or antigen concentration of the unknown samples whose OD is beyond the linear range.
date: 1992-08-30
date_type: published
publication: Journal of immunological methods
volume: 153
number: 1-2
publisher: Elsevier Science
pagerange: 263-4
refereed: TRUE
issn: 0022-1759
official_url: http://www.sciencedirect.com/science/article/pii/002217599290330V
related_url_url: http://www.sciencedirect.com/science/article/pii/002217599290330V
related_url_type: pub
citation:   Raghava, G.P.S. and Joshi, A and Agrewala, J N  (1992) Calculation of antibody and antigen concentrations from ELISA data using a graphical method.  Journal of immunological methods, 153 (1-2).  pp. 263-4.  ISSN 0022-1759     
document_url: http://crdd.osdd.net/open/114/1/raghava1992.pdf