creators_name: Nangia, Yogesh
creators_name: Kumar, Bipul
creators_name: Kaushal, Jyotsna
creators_name: Suri, C Raman
type: article
datestamp: 2013-01-31 06:16:10
lastmod: 2015-07-22 05:30:45
metadata_visibility: show
title: Palladium@gold bimetallic nanostructures as peroxidase mimic for development of sensitive fluoroimmunoassay.
ispublished: pub
subjects: QR
keywords: Bimetallic nanoparticles; Enzyme mimics;Fluoro-immunoassay;
Bensulfuron
note: Copyright of this article belongs to Elsevier Science.
abstract: In this paper, gold nanoparticles coated with palladium dots (Pd@Au) bimetallic nanostructures have been reported to have a peroxidase like activity which is not found in their monometallic counterparts. Based on this finding, we have developed an immunoassay in which antibody-modified Pd@Au nanostructure catalyzes the dimerization of a fluorogenic substrate for peroxidase, 3-(4-dihydroxy phenyl) propionic acid (HPPA), to generate high fluorescence signal. Specific antibodies against bensulfuron-methyl were generated by using a well characterized bensulfuron-protein conjugate as an immunogen, and the assay was performed in a competitive immunoassay format where Pd@Au nanostructure was bound to secondary antibody to show the peroxidase like activity. The developed immunoassay exhibited an excellent sensitivity showing a dynamic response range from 0.001 to 100ngmL(-1) for herbicide bensulfuron-methyl with a detection limit of 0.01ngmL(-1) (n=3). The newly synthesized bimetallic nanostructure shows the advantages of low cost, easy synthesis and tunable catalytic activity, making it a promising substitution of enzyme peroxidase in different applications.
date: 2012-11-02
date_type: published
publication: Analytica chimica acta
volume: 751
publisher: Elsevier Science
pagerange: 140-5
id_number: http://dx.doi.org/10.1016/j.aca.2012.09.005
refereed: TRUE
issn: 1873-4324
official_url: http://www.sciencedirect.com/science/article/pii/S0003267012013165
citation:   Nangia, Yogesh and Kumar, Bipul and Kaushal, Jyotsna and Suri, C Raman  (2012) Palladium@gold bimetallic nanostructures as peroxidase mimic for development of sensitive fluoroimmunoassay.  Analytica chimica acta, 751.  pp. 140-5.  ISSN 1873-4324     
document_url: http://crdd.osdd.net/open/1216/1/suri2012.1.pdf