creators_name: Srinivasan, Vijaya Bharathi
creators_name: Vaidyanathan, Vasanth
creators_name: Mondal, Amitabha
creators_name: Venkataramaiah, Manjunath
creators_name: Rajamohan, Govindan
type: article
datestamp: 2013-01-31 04:33:32
lastmod: 2013-01-31 04:33:32
metadata_visibility: show
title: Functional characterization of a novel Mn(2+) dependent protein serine/threonine kinase KpnK, produced by Klebsiella pneumoniae strain MGH78578.
ispublished: pub
subjects: QR
keywords:  Nosocomial pathogen; Antimicrobial resistance; Ser/Thr kinase;Klebsiella pneumoniae
note: Copyright of this article belongs to Elsevier Science.
abstract: Klebsiella pneumoniae MGH78578 contains ∼500 uncharacterized signaling proteins and in this study, we characterized the biological functions of a novel eukaryotic-like serine/threonine kinase; ESTK (KpnK). Studies demonstrated that KpnK undergoes autophosphorylation within the pH range 7.0-7.5 at 37°C in a time- and concentration- dependent manner, with Mn(2+) as its cofactor. The ΔkpnK mutant exhibited higher sensitivity to gastrointestinal and oxidative stresses. Deletion of kpnK resulted in a two to threefold increased susceptibility towards imipenem, cefepime, ceftriaxone and ceftazidime. Our study has provided overall evidence for the involvement of ESTK in regulating bacterial physiology, stress response and drug resistance. This report has unmasked the occurrence of Ser/Thr kinase mediated signaling for the first time in K. pneumoniae. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: KpnKphosphorylatesKpnK by protein kinase assay (View interaction).
date: 2012-11-02
date_type: published
publication: FEBS letters
volume: 586
number: 21
publisher: Elsevier Science
pagerange: 3778-86
refereed: TRUE
issn: 1873-3468
official_url: http://www.sciencedirect.com/science/article/pii/S0014579312007144
related_url_url: http://www.sciencedirect.com/science/article/pii/S0014579312007144
related_url_type: org
citation:   Srinivasan, Vijaya Bharathi and Vaidyanathan, Vasanth and Mondal, Amitabha and Venkataramaiah, Manjunath and Rajamohan, Govindan  (2012) Functional characterization of a novel Mn(2+) dependent protein serine/threonine kinase KpnK, produced by Klebsiella pneumoniae strain MGH78578.  FEBS letters, 586 (21).  pp. 3778-86.  ISSN 1873-3468     
document_url: http://crdd.osdd.net/open/1219/1/rajmohan2012.1.pdf