TY  - JOUR
ID  - open125
UR  - http://www.sciencedirect.com/science/article/pii/S0304416506003758
IS  - 4
A1  - Dhiman, Rohan
A1  - Raje, Manoj
A1  - Majumdar, Sekhar
N2  - The present study was conducted to see the role of NF-kappaB in virulent (Mycobacterium tuberculosis H37Rv) and avirulent (M. tuberculosis H37Ra) mycobacterial infection in THP-1 cells. To inactivate NF-kappaB, pCMV-IkappaBalphaM dn containing THP-1 cell line was generated which showed marked increase in apoptosis with M. tuberculosis H37Rv and M. tuberculosis H37Ra. Infected THP-1-IkappaBalphaM dn cells showed decrease in mitochondrial membrane potential, cytochrome c release, activation of caspase-3 and enhanced TNF-alpha production. Increase in apoptosis of infected THP-1-IkappaBalphaM dn cells resulted in inhibition of intracellular mycobacterial growth. Differential NF-kappaB activation potential was observed with M. tuberculosis H37Rv and M. tuberculosis H37Ra. Both the strains activated NF-kappaB after 4 h in THP-1 cells however after 48 h only M. tuberculosis H37Rv activated NF-kappaB which lead to up-regulation of bcl-2 family anti-apoptotic member, bfl-1/A1. Our results indicated that NF-kappaB activation may be a determinant factor for the success of virulent mycobacteria within macrophages.
VL  - 1770
TI  - Differential expression of NF-kappaB in mycobacteria infected THP-1 affects apoptosis.
AV  - restricted
EP  - 58
N1  - Copyright of this article belongs to Elsevier Science.
Y1  - 2007/04//
PB  - Elsevier Science
JF  - Biochimica et biophysica acta
KW  - THP-1 cells; Mycobacteria; NF-?B; Apoptosis; bfl-1/A1
SN  - 0006-3002
SP  - 649
ER  -