creators_name: Dhiman, Rohan
creators_name: Raje, Manoj
creators_name: Majumdar, Sekhar
type: article
datestamp: 2012-01-09 05:06:13
lastmod: 2012-01-09 05:06:13
metadata_visibility: show
title: Differential expression of NF-kappaB in mycobacteria infected THP-1 affects apoptosis.
ispublished: pub
subjects: QD
full_text_status: restricted
keywords: THP-1 cells; Mycobacteria; NF-κB; Apoptosis; bfl-1/A1
note: Copyright of this article belongs to Elsevier Science.
abstract: The present study was conducted to see the role of NF-kappaB in virulent (Mycobacterium tuberculosis H37Rv) and avirulent (M. tuberculosis H37Ra) mycobacterial infection in THP-1 cells. To inactivate NF-kappaB, pCMV-IkappaBalphaM dn containing THP-1 cell line was generated which showed marked increase in apoptosis with M. tuberculosis H37Rv and M. tuberculosis H37Ra. Infected THP-1-IkappaBalphaM dn cells showed decrease in mitochondrial membrane potential, cytochrome c release, activation of caspase-3 and enhanced TNF-alpha production. Increase in apoptosis of infected THP-1-IkappaBalphaM dn cells resulted in inhibition of intracellular mycobacterial growth. Differential NF-kappaB activation potential was observed with M. tuberculosis H37Rv and M. tuberculosis H37Ra. Both the strains activated NF-kappaB after 4 h in THP-1 cells however after 48 h only M. tuberculosis H37Rv activated NF-kappaB which lead to up-regulation of bcl-2 family anti-apoptotic member, bfl-1/A1. Our results indicated that NF-kappaB activation may be a determinant factor for the success of virulent mycobacteria within macrophages.
date: 2007-04
date_type: published
publication: Biochimica et biophysica acta
volume: 1770
number: 4
publisher: Elsevier Science
pagerange: 649-58
refereed: TRUE
issn: 0006-3002
official_url: http://www.sciencedirect.com/science/article/pii/S0304416506003758
related_url_url: http://www.sciencedirect.com/science/article/pii/S0304416506003758
related_url_type: pub
citation:   Dhiman, Rohan and Raje, Manoj and Majumdar, Sekhar  (2007) Differential expression of NF-kappaB in mycobacteria infected THP-1 affects apoptosis.  Biochimica et biophysica acta, 1770 (4).  pp. 649-58.  ISSN 0006-3002     
document_url: http://crdd.osdd.net/open/125/1/majumdar2007.pdf