…
comment: The repository administrator has not yet configured an RDF license.
primary topic: Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii.

Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii.
type: AcademicArticle
type: Article
abstract: Because of its natural ability to utilize both xylose and arabinose, the halotolerant and osmotolerant yeast Debaryomyces hansenii is considered as a potential microbial platform for exploiting lignocellulosic biomass. To gain better understanding of the xylose metabolism in D. hansenii, we have cloned and characterized a xylitol dehydrogenase gene (DhXDH). The cloned gene appeared to be essential for xylose metabolism in D. hansenii as the deletion of this gene abolished the growth of the cells on xylose. The expression of DhXDH was strongly upregulated in the presence of xylose. Recombinant DhXdhp was expressed and purified from Escherichia coli. DhXdhp was highly active against xylitol and sorbitol as substrate. Our results showed that DhXdhp was thermo-sensitive, and except this, its biochemical properties were quite comparable with XDH from other yeast species. Furthermore, to make this enzyme suitable for metabolic engineering of D. hansenii, we have improved its thermotolerance and modified cofactor requirement through modelling and mutagenesis approach.
date: 2012-04-11
is part of: Applied microbiology and biotechnology
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
has document: Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (PDF)
has version: Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (PDF)
has published: Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (PDF)
has document: Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (Other)
subject: QR Microbiology

Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (PDF)
type: Document

Springer
type: Organization

Applied microbiology and biotechnology
type: Collection
issn: 14320614

Alok K Mondal
type: Person
given name: Alok K
family name: Mondal

Dipanwita Biswas
type: Person
given name: Dipanwita
family name: Biswas

Monika Aggarwal
type: Person
given name: Monika
family name: Aggarwal

Manish Datt
type: Person
given name: Manish
family name: Datt

Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (Other)
type: Document
is version of: Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (PDF)
is volatile version of: Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (PDF)
is index codes version of: Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (PDF)

Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (Other)
type: Document
is version of: Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (PDF)
is volatile version of: Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (PDF)
islightbox thumbnail version of: Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (PDF)

Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (Other)
type: Document
is version of: Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (PDF)
is volatile version of: Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (PDF)
ispreview thumbnail version of: Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (PDF)

Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (Other)
type: Document
is version of: Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (PDF)
is volatile version of: Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (PDF)
ismedium thumbnail version of: Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (PDF)

Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (Other)
type: Document
is version of: Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (PDF)
is volatile version of: Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (PDF)
issmall thumbnail version of: Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. (PDF)

HTML Summary of #1251 Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii.
format: text/html
primary topic: Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii.

QR Microbiology
type: Concept