title: Rapid extraction and quantitative detection of the herbicide diuron in surface water by a hapten-functionalized carbon nanotubes based electrochemical analyzer.
creator: Sharma, Priyanka
creator: Bhalla, Vijayender
creator: Tuteja, Satish
creator: Kukkar, Manil
creator: Suri, C Raman
subject: QR Microbiology
description: A solid phase extraction micro-cartridge containing a non-polar polystyrene absorbent matrix was coupled with an electrochemical immunoassay analyzer (EIA) and used for the ultra-sensitive detection of the phenyl urea herbicide diuron in real samples. The EIA was fabricated by using carboxylated carbon nanotubes (CNTs) functionalized with a hapten molecule (an amine functionalized diuron derivative). Screen printed electrodes (SPE) were modified with these haptenized CNTs and specific in-house generated anti diuron antibodies were used for bio-interface development. The immunodetection was realized in a competitive electrochemical immunoassay format using alkaline phosphatase labeled secondary anti-IgG antibody. The addition of 1-naphthyl phosphate substrate resulted in the production of an electrochemically active product, 1-naphthol, which was monitored by using differential pulse voltammetry (DPV). The assay exhibited excellent sensitivity and specificity having a dynamic response range of 0.01 pg mL(-1) to 10 μg mL(-1) for diuron with a limit of detection of around 0.1 pg mL(-1) (n = 3) in standard water samples. The micro-cartridge coupled hapten-CNTs modified SPE provided an effective and efficient electrochemical immunoassay for the real-time monitoring of pesticides samples with a very high degree of sensitivity.
publisher: RSC Publishing
date: 2012-05-21
type: Article
type: PeerReviewed
relation: http://pubs.rsc.org/en/Content/ArticleLanding/2012/AN/c2an16235k
identifier:   Sharma, Priyanka and Bhalla, Vijayender and Tuteja, Satish and Kukkar, Manil and Suri, C Raman  (2012) Rapid extraction and quantitative detection of the herbicide diuron in surface water by a hapten-functionalized carbon nanotubes based electrochemical analyzer.  The Analyst, 137 (10).  pp. 2495-502.  ISSN 1364-5528     
relation: http://crdd.osdd.net/open/1256/