title: Enzymatic modifications of cephalosporins by cephalosporin acylase and other enzymes.
creator: Sonawane, Vijay Chintaman
subject: QR Microbiology
description: Semisynthetic cephalosporins are important antibacterials in clinical practice. Semisynthetic cephalosporins are manufactured by derivatizing 7-aminocephalosporanic acid (7-ACA) and its desacetylated form. Microbial enzymes such as D-amino acid oxidase, glutaryl-7-ACA acylase and cephalosporin esterase are being used as biocatalysts for the conversion of cephalosporin C (CEPH-C) to 7-ACA and its desacetylated derivatives. Recent developments in the field of enzymatic modifications of cephalosporin with special emphasis on group of enzymes called as cephalosporin acylase is discussed in this review. Aspects related to screening methods, isolation and purification, immobilization, molecular cloning, gene structure and expression and protein engineering of cephalosporin acylases have been covered. Topics pertaining to enzymatic modifications of cephalosporin by D-amino acid oxidase, cephalosporin methoxylase and beta-lactamase are also covered.
publisher: Taylor & Francis Group/Informa Healthcare
date: 2006
type: Article
type: PeerReviewed
format: application/pdf
identifier: http://crdd.osdd.net/open/146/1/sonawane2006.pdf
relation: http://informahealthcare.com/doi/abs/10.1080/07388550600718630%20
identifier:   Sonawane, Vijay Chintaman  (2006) Enzymatic modifications of cephalosporins by cephalosporin acylase and other enzymes.  Critical reviews in biotechnology, 26 (2).  pp. 95-120.  ISSN 0738-8551     
relation: http://crdd.osdd.net/open/146/