creators_name: Sonawane, Vijay Chintaman
type: article
datestamp: 2012-01-09 04:23:13
lastmod: 2012-01-10 04:58:35
metadata_visibility: show
title: Enzymatic modifications of cephalosporins by cephalosporin acylase and other enzymes.
ispublished: pub
subjects: QR
full_text_status: restricted
keywords: cephalosporin C, 7-ACA, biotransformations, D-amino acid oxidase, esterase,acylase.
note: Copyright of this article belongs to Informa Healthcare.
abstract: Semisynthetic cephalosporins are important antibacterials in clinical practice. Semisynthetic cephalosporins are manufactured by derivatizing 7-aminocephalosporanic acid (7-ACA) and its desacetylated form. Microbial enzymes such as D-amino acid oxidase, glutaryl-7-ACA acylase and cephalosporin esterase are being used as biocatalysts for the conversion of cephalosporin C (CEPH-C) to 7-ACA and its desacetylated derivatives. Recent developments in the field of enzymatic modifications of cephalosporin with special emphasis on group of enzymes called as cephalosporin acylase is discussed in this review. Aspects related to screening methods, isolation and purification, immobilization, molecular cloning, gene structure and expression and protein engineering of cephalosporin acylases have been covered. Topics pertaining to enzymatic modifications of cephalosporin by D-amino acid oxidase, cephalosporin methoxylase and beta-lactamase are also covered.
date: 2006
publication: Critical reviews in biotechnology
volume: 26
number: 2
publisher: Taylor & Francis Group/Informa Healthcare
pagerange: 95-120
refereed: TRUE
issn: 0738-8551
official_url: http://informahealthcare.com/doi/abs/10.1080/07388550600718630%20
related_url_url: http://informahealthcare.com/doi/abs/10.1080/07388550600718630%20
related_url_type: pub
citation:   Sonawane, Vijay Chintaman  (2006) Enzymatic modifications of cephalosporins by cephalosporin acylase and other enzymes.  Critical reviews in biotechnology, 26 (2).  pp. 95-120.  ISSN 0738-8551     
document_url: http://crdd.osdd.net/open/146/1/sonawane2006.pdf