creators_name: Boradia, Vishant Mahendra
creators_name: Malhotra, Himanshu
creators_name: Thakkar, Janak Shrikant
creators_name: Tillu, Vikas Ajit
creators_name: Vuppala, Bhavana
creators_name: Patil, Pravinkumar
creators_name: Sheokand, Navdeep
creators_name: Sharma, Prerna
creators_name: Chauhan, Anoop Singh
creators_name: Raje, Manoj
creators_name: Raje, Chaaya Iyengar
type: article
datestamp: 2015-01-27 09:22:51
lastmod: 2015-01-27 09:22:51
metadata_visibility: show
title: Mycobacterium tuberculosis acquires iron by cell-surface sequestration and internalization of human holo-transferrin.
ispublished: pub
subjects: QR
keywords: Biological Sciences; Cell Science; Microbiology
note: Copyright of this article belongs to NPG.
abstract: Mycobacterium tuberculosis (M.tb), which requires iron for survival, acquires this element by synthesizing iron-binding molecules known as siderophores and by recruiting a host iron-transport protein, transferrin, to the phagosome. The siderophores extract iron from transferrin and transport it into the bacterium. Here we describe an additional mechanism for iron acquisition, consisting of an M.tb protein that drives transport of human holo-transferrin into M.tb cells. The pathogenic strain M.tb H37Rv expresses several proteins that can bind human holo-transferrin. One of these proteins is the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH, Rv1436), which is present on the surface of M.tb and its relative Mycobacterium smegmatis. Overexpression of GAPDH results in increased transferrin binding to M.tb cells and iron uptake. Human transferrin is internalized across the mycobacterial cell wall in a GAPDH-dependent manner within infected macrophages.
date: 2014
date_type: published
publication: Nature communications
volume: 5
publisher: Nature Pub. Group
pagerange: 4730
refereed: TRUE
issn: 2041-1723
official_url: http://www.nature.com/ncomms/2014/140828/ncomms5730/full/ncomms5730.html
citation:   Boradia, Vishant Mahendra and Malhotra, Himanshu and Thakkar, Janak Shrikant and Tillu, Vikas Ajit and Vuppala, Bhavana and Patil, Pravinkumar and Sheokand, Navdeep and Sharma, Prerna and Chauhan, Anoop Singh and Raje, Manoj and Raje, Chaaya Iyengar  (2014) Mycobacterium tuberculosis acquires iron by cell-surface sequestration and internalization of human holo-transferrin.  Nature communications, 5.  p. 4730.  ISSN 2041-1723