@article{open1538,
          volume = {86},
          number = {15},
           month = {August},
          author = {Priyanka Sabherwal and Munish Shorie and Preeti Pathania and Shilpa Chaudhary and K K Bhasin and Vijayender Bhalla and C Raman Suri},
           title = {Hybrid aptamer-antibody linked fluorescence resonance energy transfer based detection of trinitrotoluene.},
       publisher = {ACS},
            year = {2014},
         journal = {Analytical chemistry},
           pages = {7200--4},
             url = {http://crdd.osdd.net/open/1538/},
        abstract = {Combining synthetic macromolecules and biomolecular recognition units are promising in developing novel diagnostic and analysis techniques for detecting environmental and/or clinically important substances. Fluorescence resonance energy transfer (FRET) apta-immunosensor for explosive detection is reported using 2,4,6-trinitrotoluene (TNT) specific aptamer and antibodies tagged with respective FRET pair dyes in a sandwich immunoassay format. FITC-labeled aptamer was used as a binder molecule in the newly developed apta-immunoassay format where the recognition element was specific anti-TNT antibody labeled with rhodamine isothiocyanate. The newly developed sensing platform showed excellent sensitivity with a detection limit of the order of 0.4 nM presenting a promising candidate for routine screening of TNT in samples.}
}