title: Hybrid aptamer-antibody linked fluorescence resonance energy transfer based detection of trinitrotoluene.
creator: Sabherwal, Priyanka
creator: Shorie, Munish
creator: Pathania, Preeti
creator: Chaudhary, Shilpa
creator: Bhasin, K K
creator: Bhalla, Vijayender
creator: Suri, C Raman
description: Combining synthetic macromolecules and biomolecular recognition units are promising in developing novel diagnostic and analysis techniques for detecting environmental and/or clinically important substances. Fluorescence resonance energy transfer (FRET) apta-immunosensor for explosive detection is reported using 2,4,6-trinitrotoluene (TNT) specific aptamer and antibodies tagged with respective FRET pair dyes in a sandwich immunoassay format. FITC-labeled aptamer was used as a binder molecule in the newly developed apta-immunoassay format where the recognition element was specific anti-TNT antibody labeled with rhodamine isothiocyanate. The newly developed sensing platform showed excellent sensitivity with a detection limit of the order of 0.4 nM presenting a promising candidate for routine screening of TNT in samples.
publisher: ACS
date: 2014-08-05
type: Article
type: PeerReviewed
relation: http://pubs.acs.org/doi/abs/10.1021/ac501388a
identifier:   Sabherwal, Priyanka and Shorie, Munish and Pathania, Preeti and Chaudhary, Shilpa and Bhasin, K K and Bhalla, Vijayender and Suri, C Raman  (2014) Hybrid aptamer-antibody linked fluorescence resonance energy transfer based detection of trinitrotoluene.  Analytical chemistry, 86 (15).  pp. 7200-4.  ISSN 1520-6882     
relation: http://crdd.osdd.net/open/1538/