%0 Journal Article
%@ 1520-6882
%A Sabherwal, Priyanka
%A Shorie, Munish
%A Pathania, Preeti
%A Chaudhary, Shilpa
%A Bhasin, K K
%A Bhalla, Vijayender
%A Suri, C Raman
%D 2014
%F open:1538
%I ACS
%J Analytical chemistry
%N 15
%P 7200-4
%T Hybrid aptamer-antibody linked fluorescence resonance energy transfer based detection of trinitrotoluene.
%U http://crdd.osdd.net/open/1538/
%V 86
%X Combining synthetic macromolecules and biomolecular recognition units are promising in developing novel diagnostic and analysis techniques for detecting environmental and/or clinically important substances. Fluorescence resonance energy transfer (FRET) apta-immunosensor for explosive detection is reported using 2,4,6-trinitrotoluene (TNT) specific aptamer and antibodies tagged with respective FRET pair dyes in a sandwich immunoassay format. FITC-labeled aptamer was used as a binder molecule in the newly developed apta-immunoassay format where the recognition element was specific anti-TNT antibody labeled with rhodamine isothiocyanate. The newly developed sensing platform showed excellent sensitivity with a detection limit of the order of 0.4 nM presenting a promising candidate for routine screening of TNT in samples.