TY  - JOUR
ID  - open1538
UR  - http://pubs.acs.org/doi/abs/10.1021/ac501388a
IS  - 15
A1  - Sabherwal, Priyanka
A1  - Shorie, Munish
A1  - Pathania, Preeti
A1  - Chaudhary, Shilpa
A1  - Bhasin, K K
A1  - Bhalla, Vijayender
A1  - Suri, C Raman
Y1  - 2014/08/05/
N2  - Combining synthetic macromolecules and biomolecular recognition units are promising in developing novel diagnostic and analysis techniques for detecting environmental and/or clinically important substances. Fluorescence resonance energy transfer (FRET) apta-immunosensor for explosive detection is reported using 2,4,6-trinitrotoluene (TNT) specific aptamer and antibodies tagged with respective FRET pair dyes in a sandwich immunoassay format. FITC-labeled aptamer was used as a binder molecule in the newly developed apta-immunoassay format where the recognition element was specific anti-TNT antibody labeled with rhodamine isothiocyanate. The newly developed sensing platform showed excellent sensitivity with a detection limit of the order of 0.4 nM presenting a promising candidate for routine screening of TNT in samples.
PB  - ACS
JF  - Analytical chemistry
VL  - 86
SN  - 1520-6882
TI  - Hybrid aptamer-antibody linked fluorescence resonance energy transfer based detection of trinitrotoluene.
SP  - 7200
EP  - 4
ER  -