%A Priyanka Sabherwal
%A Munish Shorie
%A Preeti Pathania
%A Shilpa Chaudhary
%A K K Bhasin
%A Vijayender Bhalla
%A C Raman Suri
%J Analytical chemistry
%T Hybrid aptamer-antibody linked fluorescence resonance energy transfer based detection of trinitrotoluene.
%X Combining synthetic macromolecules and biomolecular recognition units are promising in developing novel diagnostic and analysis techniques for detecting environmental and/or clinically important substances. Fluorescence resonance energy transfer (FRET) apta-immunosensor for explosive detection is reported using 2,4,6-trinitrotoluene (TNT) specific aptamer and antibodies tagged with respective FRET pair dyes in a sandwich immunoassay format. FITC-labeled aptamer was used as a binder molecule in the newly developed apta-immunoassay format where the recognition element was specific anti-TNT antibody labeled with rhodamine isothiocyanate. The newly developed sensing platform showed excellent sensitivity with a detection limit of the order of 0.4 nM presenting a promising candidate for routine screening of TNT in samples.
%N 15
%P 7200-4
%V 86
%D 2014
%I ACS
%L open1538