TY  - JOUR
ID  - open1571
UR  - http://www.sciencedirect.com/science/article/pii/S0223523414005583
A1  - Lohan, Sandeep
A1  - Monga, Jitender
A1  - Cameotra, Swaranjit Singh
A1  - Bisht, Gopal Singh
Y1  - 2014/12/17/
N2  - We investigated the in vitro activities of short lipopeptides against a large panel of clinical isolates of antibiotic resistant bacteria. In the animal model, LP16 (5 mg/kg) significantly decreased the burden of viable colony forming unit (CFU) of bacteria. MTT assay results revealed the high selectivity of lipopeptides toward microbial cells. Calcein dye leakage experiments and flow cytometric analysis suggests the membranolytic effect of lipopeptides, which was further confirmed by visualizing bacterial damage via electron microscopy tool (SEM & TEM). Moreover, stability in human blood plasma and no sign of resistance development against clinical isolates of Escherichia coli and Staphylococcus aureus were observed for lead lipopeptides. These results demonstrate the potential of short lipopeptides as a novel class of anti-infectives.
PB  - Paris : Editions Scientifiques Elsevier
JF  - European journal of medicinal chemistry
VL  - 88
KW  - Antibacterial activity; Antibiotic resistance; Lipopeptides; Membrane active; Plasma stability
SN  - 1768-3254
TI  - In vitro and in vivo antibacterial evaluation and mechanistic study of ornithine based small cationic lipopeptides against antibiotic resistant clinical isolates.
SP  - 19
EP  - 27
ER  -