creators_name: Manickam, Natesan
creators_name: Singh, Nitin Kumar
creators_name: Bajaj, Abhay
creators_name: Kumar, Rajendran Mathan
creators_name: Kaur, Gurwinder
creators_name: Kaur, Navjot
creators_name: Bala, Monu
creators_name: Kumar, Anand
creators_name: Mayilraj, Shanmugam
type: article
datestamp: 2015-02-10 06:11:49
lastmod: 2015-02-10 06:11:49
metadata_visibility: show
title: Bacillus mesophilum sp. nov., strain IITR-54T, a novel 4-chlorobiphenyl dechlorinating bacterium.
ispublished: pub
abstract: The taxonomic position of a Gram-positive, endospore-forming bacterium isolated from soil sample collected from an industrial site was analyzed by a polyphasic approach. The strain designated as IITR-54T matched most of the phenotypic and chemical characteristics of the genus Bacillus and represents a novel species. It was found to biodegrade 4-chlorobiphenyl through dechlorination and was isolated through enrichment procedure from an aged polychlorinated biphenyl-contaminated soil. Both resting cell assay and growth under aerobic liquid conditions using 4-chlorobiphenyl as sole source of carbon along with 0.01% yeast extract, formation of chloride ions was measured. 16S rRNA (1,489 bases) nucleotide sequence of isolated strain was compared with those of closely related Bacillus type strains and confirmed that the strain belongs to the genus Bacillus. Strain IITR-54T differs from all other species of Bacillus by at least 2.1% at the 16S rRNA level, and the moderately related species are Bacillus oceanisediminis (97.9%) followed by Bacillus infantis (97.7%), Bacillus firmus (97.4%), Bacillus drentensis (97.3%), Bacillus circulans (97.2%), Bacillus soli (97.1%), Bacillus horneckiae (97.1%), Bacillus pocheonensis (97.1%) and Bacillus bataviensis (97.1%), respectively. The cell wall peptidoglycan contained meso-diaminopimelic acid and the major isoprenoid quinone was MK-7. Major fatty acids are iso-C15:0 (32.4%) and anteiso-C15:0 (27.4%). Predominant polar lipids are diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The results of physiological and biochemical tests allowed the genotypic and phenotypic distinctiveness of strain IITR-54T with its phylogenetic relatives and suggest that the strain IITR-54T should be recognized as a novel species, for which the name Bacillus mesophilum sp. nov. is proposed. The type strain is IITR-54T (=MTCC 11060T=JCM 19208T).
date: 2014-07
date_type: published
publication: Archives of microbiology
volume: 196
number: 7
publisher: Berlin, New York, Springer-Verlag.
pagerange: 517-23
refereed: TRUE
issn: 1432-072X
official_url: http://link.springer.com/article/10.1007%2Fs00203-014-0988-9
citation:   Manickam, Natesan and Singh, Nitin Kumar and Bajaj, Abhay and Kumar, Rajendran Mathan and Kaur, Gurwinder and Kaur, Navjot and Bala, Monu and Kumar, Anand and Mayilraj, Shanmugam  (2014) Bacillus mesophilum sp. nov., strain IITR-54T, a novel 4-chlorobiphenyl dechlorinating bacterium.  Archives of microbiology, 196 (7).  pp. 517-23.  ISSN 1432-072X