<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Clathrin-mediated hemoglobin endocytosis is essential for survival of Leishmania."^^ . "Leishmania is auxotroph for heme. Previously, we have shown that Leishmania acquire heme from the degradation of endocytosed hemoglobin via a specific receptor located in the flagellar pocket. Here, we report the cloning and expression of clathrin heavy chain from Leishmania (Ld-CHC) and provide evidences that Ld-CHC is localized in flagellar pocket and regulates Hb-endocytosis in Leishmania. Kinetic analysis of Hb trafficking in GFP-Ld-CHC overexpressed Leishmania reveals that Hb is internalized through Ld-CHC coated region and remains associated with Ld-CHC containing vesicles at early time points of internalization and subsequently starts dissociating from Hb-containing vesicles at later time points indicating that clathrin-coating and uncoating regulate Hb trafficking in Leishmania. Interestingly, overexpression of dominant negative mutant of clathrin heavy chain of Leishmania (GFP-Ld-CHC-Hub) blocks the Hb internalization and causes severe growth defect in parasite. Moreover, we have shown that chlorpromazine, a pharmacological agent, blocks Hb internalization in Leishmania by depolymerizing Ld-CHC and thereby inhibits the growth of the parasites. Taken together, our results have shown that Hb endocytosis in Leishmania is a clathrin dependent process and is essential for the survival of the parasites."^^ . "2013-05" . "1833" . "5" . . "Amsterdam [etc.] Elsevier Pub. Co."^^ . . . "Biochimica et biophysica acta"^^ . . . "00063002" . . . . . . . . . . . . . . . . . . . . "Deepika"^^ . "Gupta"^^ . "Deepika Gupta"^^ . . "Ruchir"^^ . "Rastogi"^^ . "Ruchir Rastogi"^^ . . "Amitabha"^^ . "Mukhopadhyay"^^ . "Amitabha Mukhopadhyay"^^ . . "Manoj"^^ . "Raje"^^ . "Manoj Raje"^^ . . "Nitin"^^ . "Patel"^^ . "Nitin Patel"^^ . . "Shruti"^^ . "Agarwal"^^ . "Shruti Agarwal"^^ . . . . . "HTML Summary of #1606 \n\nClathrin-mediated hemoglobin endocytosis is essential for survival of Leishmania.\n\n" . "text/html" . .