creators_name: Sechi, Leonardo A.
creators_name: Jain, Shweta
creators_name: Chodisetti, Sathi Babu
creators_name: Agrewala, J N
type: article
datestamp: 2015-02-11 06:55:10
lastmod: 2015-07-22 04:33:40
metadata_visibility: show
title: CD40 Signaling Synergizes with TLR-2 in the BCR Independent Activation of Resting B Cells
ispublished: pub
abstract: Conventionally, signaling through BCR initiates sequence of events necessary for activation and differentiation of B cells. We report an alternative approach, independent of BCR, for stimulating resting B (RB) cells, by involving TLR-2 and CD40 - molecules crucial for innate and adaptive immunity. CD40 triggering of TLR-2 stimulated RB cells significantly augments their activation, proliferation and differentiation. It also substantially ameliorates the calcium flux, antigen uptake capacity and ability of B cells to activate T cells. The survival of RB cells was improved and it increases the number of cells expressing activation induced deaminase (AID), signifying class switch recombination (CSR). Further, we also observed increased activation rate and decreased threshold period required for optimum stimulation of RB cells. These results corroborate well with microarray gene expression data. This study provides novel insights into coordination between the molecules of innate and adaptive immunity in activating B cells, in a BCR independent manner. This strategy can be exploited to design vaccines to bolster B cell activation and antigen presenting efficiency, leading to faster and better immune response.
date: 2011
publication: PLoS ONE
volume: 6
number: 6
pagerange: e20651
id_number: doi:10.1371/journal.pone.0020651
refereed: TRUE
issn: 1932-6203
official_url: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0020651
citation:   Sechi, Leonardo A. and Jain, Shweta and Chodisetti, Sathi Babu and Agrewala, J N  (2011) CD40 Signaling Synergizes with TLR-2 in the BCR Independent Activation of Resting B Cells.  PLoS ONE, 6 (6).  e20651.  ISSN 1932-6203