TY  - JOUR
ID  - open1615
UR  - http://www.sciencedirect.com/science/article/pii/S0956566313000213
A1  - Chopra, Adity
A1  - Tuteja, Satish
A1  - Sachdeva, Naresh
A1  - Bhasin, K K
A1  - Bhalla, Vijayender
A1  - Suri, C Raman
Y1  - 2013/06/15/
N2  - Highly luminescent water soluble CdTe quantum dots (QDs) were synthesized and conjugated with anti-HbA1c antibody to generate specific nanobioprobe. A sandwich immunoassay model was employed using capture HbA1c antibody as a specific receptor molecule and the QD-labeled secondary antibody as a dual (fluorescence cum electrochemical) tracer to quantify the concentration of HbA1c. A linear increase in current was observed for HbA1c analytical standards with a R(2) value of 0.990 and coefficient of variance ~5%. A comparison between HPLC and dual immunoassay for clinical samples showed a correlation coefficient of 89% and 96% for fluorescence and electrochemical detection methods respectively. The QD-based immunoassay shows great promise for rapid reproducible and cost effective analysis of HbA1c in clinical samples.
PB  - Oxford : Elsevier Advanced Technology
JF  - Biosensors & bioelectronics
VL  - 44
SN  - 1873-4235
TI  - CdTe nanobioprobe based optoelectrochemical immunodetection of diabetic marker HbA1c.
SP  - 132
EP  - 5
ER  -