%0 Journal Article %@ 1466-5026 %A Singla, Arvind Kumar %A Mayilraj, Shanmugam %A Kudo, Takuji %A Krishnamurthi, S %A Prasad, G S %A Vohra, R M %D 2005 %F open:163 %I International Union of Microbiological Societies %J International journal of systematic and evolutionary microbiology %N Pt 6 %P 2561-4 %T Actinoalloteichus spitiensis sp. nov., a novel actinobacterium isolated from a cold desert of the Indian Himalayas. %U http://crdd.osdd.net/open/163/ %V 55 %X An actinobacterial strain, RMV-1378T, isolated from a cold desert of the Indian Himalayas, was subjected to polyphasic taxonomic characterization. The strain formed branching, non-fragmenting vegetative hyphae and did not produce diffusible pigments. Neither aerial mycelium nor spore formation was observed. The G+C content of the DNA was 72.0 mol%. The strain had chemotaxonomic characteristics typical of the genus Actinoalloteichus and was closely related (99.3 % 16S rRNA gene sequence similarity) to Actinoalloteichus cyanogriseus, currently the only Actinoalloteichus species with a validly published name. However, the results of DNA-DNA hybridization experiments showed 51.9 % relatedness with the type strain of A. cyanogriseus. On the basis of the above data and the physiological and biochemical distinctiveness of RMV-1378T (=MTCC 6194T=JCM 12472T=DSM 44848T), this strain should be classified as the type strain of a novel species of Actinoalloteichus, for which the name Actinoalloteichus spitiensis sp. nov. is proposed. %Z Copyright of this article belongs to International Union of Microbiological Societies.