TY  - JOUR
N1  - Copyright of this article belongs to ASM.
ID  - open1661
UR  - http://aac.asm.org/content/early/2015/02/10/AAC.03266-14.full.pdf
IS  - 5
A1  - Bhatia, Rakesh
A1  - Gautam, Ankur
A1  - Gautam, Shailendra K
A1  - Mehta, Divya
A1  - Kumar, Vinod
A1  - Raghava, G.P.S.
A1  - Varshney, Grish C
Y1  - 2015/05//
N2  - The SYBR green I (SG) dye-based fluorescence assay for screening antimalarial compounds is based on direct quantitation of parasite DNA. We show that DNA-interacting cationic cell-penetrating peptides (CPPs) and intercalating agents compete with SG dye to bind to DNA. Therefore, readouts of this assay, unlike those of the [(3)H]hypoxanthine incorporation assay, for the antimalarial activity of the above DNA binding agents may be erroneous. In the case of CPPs, false readouts can be improved by the removal of excess peptides.
PB  - American Society for Microbiology
JF  - Antimicrobial agents and chemotherapy
VL  - 59
KW  - Bioinformatics; DNA Intercalating Agents
SN  - 1098-6596
TI  - Assessment of SYBR green I dye-based fluorescence assay for screening antimalarial activity of cationic peptides and DNA intercalating agents.
SP  - 2886
EP  - 9
ER  -