@article{open1685,
          volume = {64},
          number = {Pt 8},
           month = {August},
          author = {Syed G Dastager and Rahul Mawlankar and Shan-Kun Tang and S Krishnamurthi and V Venkata Ramana and Neeta Joseph and Yogesh S Shouche},
            note = {Copyright of this article belongs to Society for General Microbiology},
           title = {Rhodococcus enclensis sp. nov., a novel member of the genus Rhodococcus.},
       publisher = {Society for General Microbiology},
            year = {2014},
         journal = {International journal of systematic and evolutionary microbiology},
           pages = {2693--9},
        keywords = {Genus Rhodococcus},
             url = {http://crdd.osdd.net/open/1685/},
        abstract = {A novel actinobacterial strain, designated, NIO-1009(T), was isolated from a marine sediment sample collected from Chorao Island, Goa, India. Phylogenetic analysis comparisons based on 16S rRNA gene sequences between strain NIO-1009(T) and other members of the genus Rhodococcus revealed that strain NIO-1009(T) had the closest sequence similarity to Rhodococcus kroppenstedtii DSM 44908(T) and Rhodococcus corynebacterioides DSM 20151(T) with 99.2 and 99.1\%, respectively. Furthermore, DNA-DNA hybridization results showed that R. kroppenstedtii DSM 44908(T) and R. corynebacterioides DSM 20151(T) were 39.5 (3.0\%) and 41.7 (2.0\%) with strain NIO-1009(T), respectively, which were well below the 70\% limit for any novel species proposal. Phylogenetically strain NIO-1009(T) forms a stable clade with and R. kroppenstedtii DSM 44908(T) and R. corynebacterioides DSM 20151(T) with 100\% bootstrap values. Strain NIO-1009(T) contained meso-diaminopimelic acid as the diagnostic diamino acid and galactose and arabinose as the cell wall sugars. The major fatty acids were C(16 : 0), C(18 : 1){\ensuremath{\omega}}9c, C(16 : 1)({\ensuremath{\omega}}6c and/or {\ensuremath{\omega}}7c) and 10-methyl C(18 : 0). The only menaquinone detected was MK-8(H2), while the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and one unknown phospholipid. The G+C content of the genomic DNA was 66.9 mol\%. The phenotypic and genotypic data showed that strain NIO-1009(T) warrants recognition as a novel species of the genus Rhodococcus for which the name Rhodococcus enclensis sp. nov., is proposed; the type strain is NIO-1009(T) ( = NCIM 5452(T) = DSM 45688(T)).}
}