%A Syed G Dastager
%A Rahul Mawlankar
%A Shan-Kun Tang
%A S Krishnamurthi
%A V Venkata Ramana
%A Neeta Joseph
%A Yogesh S Shouche
%O Copyright of this article belongs to Society for General Microbiology
%J International journal of systematic and evolutionary microbiology
%T Rhodococcus enclensis sp. nov., a novel member of the genus Rhodococcus.
%X A novel actinobacterial strain, designated, NIO-1009(T), was isolated from a marine sediment sample collected from Chorao Island, Goa, India. Phylogenetic analysis comparisons based on 16S rRNA gene sequences between strain NIO-1009(T) and other members of the genus Rhodococcus revealed that strain NIO-1009(T) had the closest sequence similarity to Rhodococcus kroppenstedtii DSM 44908(T) and Rhodococcus corynebacterioides DSM 20151(T) with 99.2 and 99.1%, respectively. Furthermore, DNA-DNA hybridization results showed that R. kroppenstedtii DSM 44908(T) and R. corynebacterioides DSM 20151(T) were 39.5 (3.0%) and 41.7 (2.0%) with strain NIO-1009(T), respectively, which were well below the 70% limit for any novel species proposal. Phylogenetically strain NIO-1009(T) forms a stable clade with and R. kroppenstedtii DSM 44908(T) and R. corynebacterioides DSM 20151(T) with 100% bootstrap values. Strain NIO-1009(T) contained meso-diaminopimelic acid as the diagnostic diamino acid and galactose and arabinose as the cell wall sugars. The major fatty acids were C(16?:?0), C(18?:?1)?9c, C(16?:?1)(?6c and/or ?7c) and 10-methyl C(18?:?0). The only menaquinone detected was MK-8(H2), while the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and one unknown phospholipid. The G+C content of the genomic DNA was 66.9 mol%. The phenotypic and genotypic data showed that strain NIO-1009(T) warrants recognition as a novel species of the genus Rhodococcus for which the name Rhodococcus enclensis sp. nov., is proposed; the type strain is NIO-1009(T) (?=?NCIM 5452(T)?=?DSM 45688(T)).
%N Pt 8
%K Genus Rhodococcus
%P 2693-9
%V 64
%D 2014
%I Society for General Microbiology
%L open1685