@article{open1719, volume = {9}, number = {3}, month = {June}, author = {Markus G{\"o}ker and Megan Lu and Anne Fiebig and Matt Nolan and Alla Lapidus and Hope Tice and Tijana Glavina Del Rio and Jan-Fang Cheng and Cliff Han and Roxanne Tapia and Lynne A Goodwin and Sam Pitluck and Konstantinos Liolios and Konstantinos Mavromatis and Ioanna Pagani and Natalia Ivanova and Natalia Mikhailova and Amrita Pati and Amy Chen and Krishna Palaniappan and Miriam Land and Shanmugam Mayilraj and Manfred Rohde and John C Detter and Boyke Bunk and Stefan Spring and Reinhard Wirth and Tanja Woyke and James Bristow and Jonathan A Eisen and Victor Markowitz and Philip Hugenholtz and Nikos C Kyrpides and Hans-Peter Klenk}, note = {Open Access}, title = {Genome sequence of the mud-dwelling archaeon Methanoplanus limicola type strain (DSM 2279(T)), reclassification of Methanoplanus petrolearius as Methanolacinia petrolearia and emended descriptions of the genera Methanoplanus and Methanolacinia.}, publisher = {Standard Genomics Consortium}, year = {2014}, journal = {Standards in genomic sciences}, pages = {1076--88}, keywords = {GEBA; Methanomicrobiaceae; anaerobic; improved-high-quality draft; mesophilic; methanogen; motile; swamp}, url = {http://crdd.osdd.net/open/1719/}, abstract = {Methanoplanus limicola Wildgruber et al. 1984 is a mesophilic methanogen that was isolated from a swamp composed of drilling waste near Naples, Italy, shortly after the Archaea were recognized as a separate domain of life. Methanoplanus is the type genus in the family Methanoplanaceae, a taxon that felt into disuse since modern 16S rRNA gene sequences-based taxonomy was established. Methanoplanus is now placed within the Methanomicrobiaceae, a family that is so far poorly characterized at the genome level. The only other type strain of the genus with a sequenced genome, Methanoplanus petrolearius SEBR 4847(T), turned out to be misclassified and required reclassification to Methanolacinia. Both, Methanoplanus and Methanolacinia, needed taxonomic emendations due to a significant deviation of the G+C content of their genomes from previously published (pre-genome-sequence era) values. Until now genome sequences were published for only four of the 33 species with validly published names in the Methanomicrobiaceae. Here we describe the features of M. limicola, together with the improved-high-quality draft genome sequence and annotation of the type strain, M3(T). The 3,200,946 bp long chromosome (permanent draft sequence) with its 3,064 protein-coding and 65 RNA genes is a part of the G enomic E ncyclopedia of B acteria and Archaea project.} }