TY  - JOUR
N1  - Copyright of this article belongs to NPG.
ID  - open1733
UR  - http://www.nature.com/articles/srep18465
A1  - Chauhan, Anoop S
A1  - Rawat, Pooja
A1  - Malhotra, Himanshu
A1  - Sheokand, Navdeep
A1  - Kumar(1), Manoj
A1  - Patidar, Anil
A1  - Chaudhary, Surbhi
A1  - Jakhar, Priyanka
A1  - Raje, Chaaya I
A1  - Raje, Manoj
Y1  - 2015///
N2  - Lactoferrin is a crucial nutritionally important pleiotropic molecule and iron an essential trace metal for all life. The current paradigm is that living organisms have evolved specific membrane anchored receptors along with iron carrier molecules for regulated absorption, transport, storage and mobilization of these vital nutrients. We present evidence for the existence of non-canonical pathway whereby cells actively forage these vital resources from beyond their physical boundaries, by secreting the multifunctional housekeeping enzyme Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) into the extracellular milieu. This effect's an autocrine/paracrine acquisition of target ligand into the cell. Internalization by this route is extensively favoured even by cells that express surface receptors for lactoferrin and involves urokinase plasminogen activator receptor (uPAR). We also demonstrate the operation of this phenomenon during inflammation, as an arm of the innate immune response where lactoferrin denies iron to invading microorganisms by chelating it and then itself being sequestered into surrounding host cells by GAPDH.
PB  - NPG
JF  - Scientific reports
VL  - 5
KW  - Glyceraldehyde-3-phosphate
SN  - 2045-2322
TI  - Secreted multifunctional Glyceraldehyde-3-phosphate dehydrogenase sequesters lactoferrin and iron into cells via a non-canonical pathway.
ER  -