%A Praveen Sonkusre
%A Swaranjit Singh Cameotra
%O Copyright of this article belongs to Elsevier Science.
%J Colloids and surfaces. B, Biointerfaces
%T Biogenic selenium nanoparticles inhibit Staphylococcus aureus adherence on different surfaces.
%X The global issue of nosocomial infection is owing to bacterial colonization and biofilm formation on medical devices which primarily affects critically ill and/or immuno-compromised patients and also leads to malfunctioning of the devices. Therefore, it is desirable to prevent bacterial colonization on these devices by coating with a non toxic antimicrobial agent or bacterial adherence inhibitor. Here we have shown Bacillus licheniformis JS2 derived selenium nanoparticles (SeNPs) inhibit Staphylococcus aureus adherence and micro-colony formation on polystyrene, glass, and catheter surface. Results indicated that, the coating of these non toxic biogenic SeNPs, at a concentration of 0.5mgSe/ml, prohibits bacterial load to more than 60% on glass and catheter surface, when incubated at 4?C for 24h in phosphate buffered saline. Furthermore, confocal and electron microscopic observations strongly suggested the inhibition of biofilm and micro-colony formation on SeNP coated glass and catheter surfaces when cultured at 37?C for 72h in a nutrient rich medium. The study suggests that coating of biogenic SeNPs on medical devices could be an alternative approach for prevention of biofilm related infections.
%K Adherence; Bacillus licheniformis JS2; Biofilm formation; Micro-colony; Selenium nanoparticles; Staphylococcus aureus
%P 1051-7
%V 136
%D 2015
%I Elsevier Science
%L open1738