creators_name: Baindara, Piyush
creators_name: Chaudhry, Vasvi
creators_name: Mittal, Garima
creators_name: Liao, Luciano M
creators_name: Matos, Carolina O
creators_name: Khatri, Neeraj
creators_name: Franco, Octavio L
creators_name: Patil, Prabhu B
creators_name: Korpole, Suresh
type: article
datestamp: 2016-01-29 06:49:21
lastmod: 2016-01-29 06:49:21
metadata_visibility: show
title: Characterization of the Antimicrobial Peptide Penisin, a Class Ia Novel Lantibiotic from Paenibacillus sp. Strain A3.
ispublished: pub
subjects: QR
note: Copyright of this article belongs to ASM.
abstract: Attempts to isolate novel antimicrobial peptides from microbial sources have been on the rise recently, despite their low efficacy in therapeutic applications. Here, we report identification and characterization of a new efficient antimicrobial peptide from a bacterial strain designated A3 that exhibited highest identity with Paenibacillus ehimensis. Upon purification and subsequent molecular characterization of the antimicrobial peptide, referred to as penisin, we found the peptide to be a bacteriocin-like peptide. Consistent with these results, RAST analysis of the entire genome sequence revealed the presence of a lantibiotic gene cluster containing genes necessary for synthesis and maturation of a lantibiotic. While circular dichroism and one-dimension nuclear magnetic resonance experiments confirmed a random coil structure of the peptide, similar to other known lantibiotics, additional biochemical evidence suggests posttranslational modifications of the core peptide yield six thioether cross-links. The deduced amino acid sequence of the putative biosynthetic gene penA showed approximately 74% similarity with elgicin A and 50% similarity with the lantibiotic paenicidin A. Penisin effectively killed methicillin-resistant Staphylococcus aureus (MRSA) and did not exhibit hemolysis activity. Unlike other lantibiotics, it effectively inhibited the growth of Gram-negative bacteria. Furthermore, 80 mg/kg of body weight of penisin significantly reduced bacterial burden in a mouse thigh infection model and protected BALB/c mice in a bacteremia model entailing infection with Staphylococcus aureus MTCC 96, suggesting that it could be a promising new antimicrobial peptide.
date: 2015
date_type: published
publication: Antimicrobial agents and chemotherapy
volume: 60
number: 1
publisher: ASM
pagerange: 580-91
refereed: TRUE
issn: 1098-6596
official_url: http://aac.asm.org/content/60/1/580.long
citation:   Baindara, Piyush and Chaudhry, Vasvi and Mittal, Garima and Liao, Luciano M and Matos, Carolina O and Khatri, Neeraj and Franco, Octavio L and Patil, Prabhu B and Korpole, Suresh  (2015) Characterization of the Antimicrobial Peptide Penisin, a Class Ia Novel Lantibiotic from Paenibacillus sp. Strain A3.  Antimicrobial agents and chemotherapy, 60 (1).  pp. 580-91.  ISSN 1098-6596