%0 Journal Article
%@ 1432-072X
%A Mawlankar, Rahul
%A Thorat, Meghana N
%A Krishnamurthi, Srinivasan
%A Dastager, Syed G
%D 2016
%F open:1752
%I Springer
%J Archives of microbiology
%K Bacillus sp; Cellulase; Marine sediment; Polyphasic taxonomy
%N 1
%P 83-9
%T Bacillus cellulasensis sp. nov., isolated from marine sediment.
%U http://crdd.osdd.net/open/1752/
%V 198
%X A novel bacterial strain NIO-1130(T) was isolated from sediment sample taken from Chorao Island, Goa Province, India, and subjected to a taxonomic investigation. The strain was Gram-positive, aerobic, and motile. Phylogenetic analysis based on 16S rRNA gene sequences placed the isolate within the genus Bacillus and strain NIO-1130(T) showed highest sequence similarity with Bacillus halosaccharovorans DSM 25387(T) (98.4 %) and Bacillus niabensis CIP 109816(T) (98.1 %), whereas other Bacillus species showed <97.0 % similarity. Tree based on gyrB gene sequence revealed that strain bacillus group. The major menaquinone was MK-7 and the predominant cellular fatty acids were iso-C15:0, anteiso-C15:0, iso-C17:0, and anteiso-C17:0. The strain showed a DNA G+C content of 39.9 mol%. DNA-DNA hybridization studies revealed that strain NIO-1130(T) exhibits 70 % similarity with Bacillus halosaccharovorans DSM 25387(T) and Bacillus niabensis CIP 109816(T). On the basis of physiological, biochemical, chemotaxonomic and phylogenetic analyses, we consider the isolate to represent a novel species of the genus Bacillus, for which the name Bacillus cellulasensis sp. nov., is proposed. The type strain is NIO-1130(T) (=NCIM 5461(T)=CCTCC AB 2011126(T)).
%Z Copyright of this article belongs to Springer.