creators_name: Mawlankar, Rahul
creators_name: Thorat, Meghana N
creators_name: Krishnamurthi, Srinivasan
creators_name: Dastager, Syed G
type: article
datestamp: 2016-01-29 04:40:43
lastmod: 2016-09-29 07:35:48
metadata_visibility: show
title: Bacillus cellulasensis sp. nov., isolated from marine sediment.
ispublished: pub
subjects: QR
keywords: Bacillus sp; Cellulase; Marine sediment; Polyphasic taxonomy
note: Copyright of this article belongs to Springer.
abstract: A novel bacterial strain NIO-1130(T) was isolated from sediment sample taken from Chorao Island, Goa Province, India, and subjected to a taxonomic investigation. The strain was Gram-positive, aerobic, and motile. Phylogenetic analysis based on 16S rRNA gene sequences placed the isolate within the genus Bacillus and strain NIO-1130(T) showed highest sequence similarity with Bacillus halosaccharovorans DSM 25387(T) (98.4 %) and Bacillus niabensis CIP 109816(T) (98.1 %), whereas other Bacillus species showed <97.0 % similarity. Tree based on gyrB gene sequence revealed that strain bacillus group. The major menaquinone was MK-7 and the predominant cellular fatty acids were iso-C15:0, anteiso-C15:0, iso-C17:0, and anteiso-C17:0. The strain showed a DNA G+C content of 39.9 mol%. DNA-DNA hybridization studies revealed that strain NIO-1130(T) exhibits 70 % similarity with Bacillus halosaccharovorans DSM 25387(T) and Bacillus niabensis CIP 109816(T). On the basis of physiological, biochemical, chemotaxonomic and phylogenetic analyses, we consider the isolate to represent a novel species of the genus Bacillus, for which the name Bacillus cellulasensis sp. nov., is proposed. The type strain is NIO-1130(T) (=NCIM 5461(T)=CCTCC AB 2011126(T)).
date: 2016-01
date_type: published
publication: Archives of microbiology
volume: 198
number: 1
publisher: Springer
pagerange: 83-9
refereed: TRUE
issn: 1432-072X
official_url: http://link.springer.com/article/10.1007%2Fs00203-015-1155-7
citation:   Mawlankar, Rahul and Thorat, Meghana N and Krishnamurthi, Srinivasan and Dastager, Syed G  (2016) Bacillus cellulasensis sp. nov., isolated from marine sediment.  Archives of microbiology, 198 (1).  pp. 83-9.  ISSN 1432-072X     
document_url: http://crdd.osdd.net/open/1752/1/56.pdf