title: Marine microbe as nano-factories for copper biomineralization creator: Kaur, Harneet creator: Dolma, Kunzes creator: Kaur, Navjot creator: Malhotra, Ankit creator: Kumar, Navinder creator: Dixit, Pooja creator: Sharma, Deepak creator: Mayilraj, S. creator: Choudhury, Anirban Roy subject: QR Microbiology description: The present research work highlighted the possibility of exploiting marine microbes for the biosynthesis of copper nanoparticles and further investigated thermodynamic changes during the biomineralization of copper in a microbial system by isothermal titration calorimetry. A bacterial strain (M-7) isolated from Kanyakumari coast, India was found capable of synthesizing these nanoparticles. Based on phylogenetic relationship, the strain M-7 was identified as Kocuria flava. The nanoparticles produced were characterized using UV-vis spectrophotometry, Transmission electron microscopy (TEM) and Dynamic light scattering (DLS). Data revealed the formation of spherical and quasi-spherical shaped nanoparticles with an average particle size ranging between 5 and 30 nm. Additionally, a study on the effect of different media components indicated that media containing higher amount of casein enzymic hydrolysate and yeast extract supported the formation of stable colloidal suspension of nanoparticles. Finally, isothermal titration calorimetry (ITC) was carried out to understand the change in heat energy during the formation of nanoparticles in different media. Combinatorial observations of all these studies may open up new strategies to develop tailor made copper nanoparticles via green route. publisher: Springer date: 2015 type: Article type: PeerReviewed relation: http://dx.doi.org/10.1007/s12257-014-0432-7 identifier: Kaur, Harneet and Dolma, Kunzes and Kaur, Navjot and Malhotra, Ankit and Kumar, Navinder and Dixit, Pooja and Sharma, Deepak and Mayilraj, S. and Choudhury, Anirban Roy (2015) Marine microbe as nano-factories for copper biomineralization. Biotechnology and Bioprocess Engineering, 20 (1). pp. 51-57. ISSN 1226-8372 relation: http://crdd.osdd.net/open/1793/