creators_name: Midha, Samriti
creators_name: Bansal, Kanika
creators_name: Sharma, Shikha
creators_name: Kumar, Narinder
creators_name: Patil, Prashant P
creators_name: Chaudhry, Vasvi
creators_name: Patil, Prabhu B
type: article
datestamp: 2016-09-30 05:53:14
lastmod: 2016-09-30 05:53:14
metadata_visibility: show
title: Genomic Resource of Rice Seed Associated Bacteria.
ispublished: pub
subjects: QR
keywords: NGS; diversity; genomics; plant-microbe interactions; rice seeds
note: Open Access
abstract: Plants are host to diverse microbiome that might have co-evolved since millions of years. This resident microbiota can act as extended genome by contributing in plant growth, development and protection from biotic and abiotic stresses. Rice (Oryza Sativa) is a staple food consumed by more than 50% of the world's population. Herein we targeted the bacterial community associated with the healthy rice seeds. In this direction, we isolated and carried out whole genome sequencing of 100 bacterial isolates. These isolates belong to three major bacterial phyla Proteobacteria, Firmicutes, and Actinobacteria that spread over 15 distinct genus and 29 species. A phylogenetic tree based on a broad set of phylogenomic marker genes confirmed the evolutionary relationship amongst the strains and their phylogenetic grouping. Average Nucleotide Identity was also used to establish species identity of isolates that form a particular phylogenetic and taxonomic grouping. The data generated from the present study is one of the first major genomic resources in the field of phytobiome research. Whole genome sequence of the members will be invaluable in this era of big data driven research. Moreover, the majority of genus and species identified in this study are already known for plant probiotic properties. This genomic data with annotation will aid in comparative, evolutionary and ecological studies of bacteria associated with plants or multi-kingdom bacteria associated with nosocomial infections.
date: 2015
date_type: published
publication: Frontiers in microbiology
volume: 6
publisher: Frontiers Research Foundation
pagerange: 1551
refereed: TRUE
issn: 1664-302X
official_url: http://journal.frontiersin.org/article/10.3389/fmicb.2015.01551/full
citation:   Midha, Samriti and Bansal, Kanika and Sharma, Shikha and Kumar, Narinder and Patil, Prashant P and Chaudhry, Vasvi and Patil, Prabhu B  (2015) Genomic Resource of Rice Seed Associated Bacteria.  Frontiers in microbiology, 6.  p. 1551.  ISSN 1664-302X     
document_url: http://crdd.osdd.net/open/1838/1/57.pdf