%A Abhay Bajaj
%A Anand Kumar
%A Shivani Yadav
%A Gurwinder Kaur
%A Monu Bala
%A Nitin Kumar Singh
%A Rajendran Mathan Kumar
%A Natesan Manickam
%A Shanmugam Mayilraj
%O Copyright of this article belongs to SGM.
%J International journal of systematic and evolutionary microbiology
%T Isolation and characterization of a novel Gram-negative bacterium Chromobacterium alkanivorans sp. nov., strain IITR-71T degrading halogenated alkanes.
%X The taxonomic position of a Gram-stain-negative, non-violacein pigmented bacterium isolated from an insecticide contaminated site was characterized by a polyphasic approach. The bacterium was able to grow on three different halogenated compounds namely 1-Chlorobutane, 1-Chloropropane and 1, 2-Dichlorethane. As a critical step in the degradation of these haloalkanes, stoichiometric amounts of dechlorination were estimated. Based on selective enrichment method for three months, using a highly contaminated mixed chemical soil, a bacterium was obtained and designated as IITR-71T. Its versatility and novelty led us to further characterize by polyphasic taxonomy. The 16S rRNA gene sequence (1446 bases) comparison showed highest similarity with those of genus Chromobacterium with the closely related species to strain IITR-71T are Chromobacterium aquaticum (99.3%) followed by Chromobacterium haemolyticum (98.6%) and Chromobacterium piscinae (97.1%). The major ubiquinone was Q-8. Predominant polar lipids are phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG). The DNA G+C content of strain IITR-71T was estimated to be 61.2 mol%. The results of genotypic and phenotypic distinctiveness of the strain IITR-71T with its phylogenetic relationship suggests that the strain IITR-71T is a novel species, for which the name Chromobacterium alkanivorans sp. nov. is proposed. The type strain is IITR-71T (= MTCC 11059T = JCM 30068T = KCTC 52433T).
%D 2016
%I Society for General Microbiology
%L open1854