creators_name: Honnavar, Prasanna
creators_name: Chakrabarti, Arunaloke
creators_name: Prasad, Ghandam S
creators_name: Singh, Pankaj
creators_name: Dogra, Sunil
creators_name: Rudramurthy, Shivaprakash M
type: article
datestamp: 2016-09-29 10:24:33
lastmod: 2016-09-29 10:24:33
metadata_visibility: show
title: β-Endorphin enhances the phospholipase activity of the dandruff causing fungi Malassezia globosa and Malassezia restricta.
ispublished: pub
subjects: QR
keywords: Malassezia, dandruff; phospholipase activity; β-endorphin
note: copyright of this article belongs to OUP.
abstract: β-Endorphin is known to stimulate phospholipase production by Malassezia pachydermatis during canine dermatoses. The role of β-endorphin in Malassezia infection in humans is not well studied. The present study compares the influence of β-endorphin on Malassezia globosa and Malassezia restricta isolated from patients with seborrhoeic dermatitis/dandruff (SD/D) and healthy controls. Malassezia isolates (five each of the two species from patients and healthy controls) were grown on modified Dixon's agar with or without 100 nmol/L β-endorphin. Phospholipase activity was quantified based on its ability to hydrolyze L-α-phosphatidylcholine dimyristoyl (phospholipid substrate). Free fatty acid was measured by a colorimetry method. In isolates from patients, the phospholipase activity significantly increased after exposure to β-endorphin (M. globosa, P = .04; M. restricta, P = .001), which did not occur in isolates from healthy controls. Moreover, after β-endorphin exposure the patient isolates had significantly higher (P = .0004) phospholipase activity compared to the healthy control isolates. The results suggest that isolates of M. globosa and M. restricta from patients may differ from those of healthy humans.
date: 2016-08-05
date_type: published
publication: Medical mycology
publisher: OUP
refereed: TRUE
issn: 1460-2709
citation:   Honnavar, Prasanna and Chakrabarti, Arunaloke and Prasad, Ghandam S and Singh, Pankaj and Dogra, Sunil and Rudramurthy, Shivaprakash M  (2016) β-Endorphin enhances the phospholipase activity of the dandruff causing fungi Malassezia globosa and Malassezia restricta.  Medical mycology.   ISSN 1460-2709