creators_name: Nallabelli, Nayudu
creators_name: Patil, Prashant P
creators_name: Pal, Vijay Kumar
creators_name: Singh, Namrata
creators_name: Jain, Ashish
creators_name: Patil, Prabhu B
creators_name: Grover, Vishakha
creators_name: Korpole, Suresh
type: article
datestamp: 2016-09-29 07:23:32
lastmod: 2016-09-29 07:23:32
metadata_visibility: show
title: Biochemical and genome sequence analyses of Megasphaera sp. strain DISK18 from dental plaque of a healthy individual reveals commensal lifestyle.
ispublished: pub
subjects: QR
keywords: Biotechnology; Microbiology
note: Open Access
abstract: Much of the work in periodontal microbiology in recent years has focused on identifying and understanding periodontal pathogens. As the majority of oral microbes have not yet been isolated in pure form, it is essential to understand the phenotypic characteristics of microbes to decipher their role in oral environment. In this study, strain DISK18 was isolated from gingival sulcus and identified as a Megasphaera species. Although metagenomics studies revealed Megasphaera species as a major group within the oral habitat, they have never been isolated in cultivable form to date. Therefore, we have characterized the DISK18 strain to better understand its role in the periodontal ecosystem. Strain Megasphaera sp. DISK18 displayed the ability to adhere and self-aggregate, which are essential requisite features for inhabiting and persisting in oral cavity. It also coaggregated with other pioneer oral colonizers like Streptococcus and Lactobacillus species but not with Veillonella. This behaviour points towards its role in the ecologic succession of a multispecies biofilm as an early colonizer. The absence of virulence determining genes as observed in whole genome sequence analysis coupled with an inability to degrade collagen reveals that Megasphaera sp. strain DISK18 is likely not a pathogenic species and emphasizes its commensal lifestyle.
date: 2016
date_type: published
publication: Scientific reports
volume: 6
publisher: NPG
pagerange: 33665
refereed: TRUE
issn: 2045-2322
official_url: http://www.nature.com/articles/srep33665
citation:   Nallabelli, Nayudu and Patil, Prashant P and Pal, Vijay Kumar and Singh, Namrata and Jain, Ashish and Patil, Prabhu B and Grover, Vishakha and Korpole, Suresh  (2016) Biochemical and genome sequence analyses of Megasphaera sp. strain DISK18 from dental plaque of a healthy individual reveals commensal lifestyle.  Scientific reports, 6.  p. 33665.  ISSN 2045-2322     
document_url: http://crdd.osdd.net/open/1893/1/62.pdf