%A Anu Priyanka
%A Vipul Solanki
%A Raman Parkesh
%A Krishan Gopal Thakur
%O Open Access
%J Proteins
%T Crystal structure of the N-terminal domain of human SIRT7 reveals a three-helical domain architecture.
%X Human SIRT7 is an NAD(+) dependent deacetylase, which belongs to sirtuin family of proteins. SIRT7, like other sirtuins has conserved catalytic domain and is flanked by N- and C-terminal domains reported to play vital functional roles. Here, we report the crystal structure of the N-terminal domain of human SIRT7 (SIRT7(NTD) ) at 2.3 ? resolution as MBP-SIRT7(NTD) fusion protein. SIRT7(NTD) adopts three-helical domain architecture and comparative structural analyses suggest similarities to some DNA binding motifs and transcription regulators. We also report here the importance of N- and C-terminal domains in soluble expression of SIRT7. Proteins 2016; 84:1558-1563. ? 2016 Wiley Periodicals, Inc.
%N 10
%K Human SIRT7; histone deacetylase; maltose binding protein; peptide mass fingerprinting; sirtuins; x-ray crystallography
%P 1558-63
%V 84
%D 2016
%I Wiley
%L open1897