title: Inhibition of preS1-hepatocyte interaction by an array of recombinant human antibodies from naturally recovered individuals.
creator: Sankhyan, Anurag
creator: Sharma, Chandresh
creator: Dutta, Durgashree
creator: Sharma, Tarang
creator: Chosdol, Kunzang
creator: Wakita, Takaji
creator: Watashi, Koichi
creator: Awasthi, Amit
creator: Acharya, Subrat K
creator: Khanna, Navin
creator: Tiwari, Ashutosh
creator: Sinha, Subrata
subject: QR Microbiology
description: Neutralizing monoclonal antibodies are being found to be increasingly useful in viral infections. In hepatitis B infection, antibodies are proven to be useful for passive prophylaxis. The preS1 region (21-47a.a.) of HBV contains the viral hepatocyte-binding domain crucial for its attachment and infection of hepatocytes. Antibodies against this region are neutralizing and are best suited for immune-based neutralization of HBV, especially in view of their not recognizing decoy particles. Anti-preS1 (21-47a.a.) antibodies are present in serum of spontaneously recovered individuals. We generated a phage-displayed scFv library using circulating lymphocytes from these individuals and selected four preS1-peptide specific scFvs with markedly distinct sequences from this library. All the antibodies recognized the blood-derived and recombinant preS1 containing antigens. Each scFv showed a discrete binding signature, interacting with different amino acids within the preS1-peptide region. Ability to prevent binding of the preS1 protein (N-terminus 60a.a.) to HepG2 cells stably expressing hNTCP (HepG2-hNTCP-C4 cells), the HBV receptor on human hepatocytes was taken as a surrogate marker for neutralizing capacity. These antibodies inhibited preS1-hepatocyte interaction individually and even better in combination. Such a combination of potentially neutralizing recombinant antibodies with defined specificities could be used for preventing/managing HBV infections, including those by possible escape mutants.
publisher: NPG
date: 2016
type: Article
type: PeerReviewed
format: application/pdf
identifier: http://crdd.osdd.net/open/1900/1/74.pdf
relation: http://www.nature.com/articles/srep21240
identifier:   Sankhyan, Anurag and Sharma, Chandresh and Dutta, Durgashree and Sharma, Tarang and Chosdol, Kunzang and Wakita, Takaji and Watashi, Koichi and Awasthi, Amit and Acharya, Subrat K and Khanna, Navin and Tiwari, Ashutosh and Sinha, Subrata  (2016) Inhibition of preS1-hepatocyte interaction by an array of recombinant human antibodies from naturally recovered individuals.  Scientific reports, 6.  p. 21240.  ISSN 2045-2322     
relation: http://crdd.osdd.net/open/1900/