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primary topic: Characterization of heat induced spherulites of lysozyme reveals new insight on amyloid initiation.

Characterization of heat induced spherulites of lysozyme reveals new insight on amyloid initiation.
type: AcademicArticle
type: Article
abstract: Here, we report results obtained during our experiments to visualize how heat transforms globular protein, lysozyme into building block of β-amyloids. Light scattering experiments showed formation of lower order associated species around 50-70 °C followed by rapid cooperativity to β-amyloid fibrils. Interestingly, crystallization drops set at higher temperatures either led to aggregates or spherulites. The latter possess an amorphous β-fibril rich core with thin crystalline needles projecting outwards. Diffraction of the crystalline outgrowths revealed novel dimers and trimers of lysozyme where individual chains were similar to monomer with marginal gain in β-sheet content. Importantly, analysis of Amide I stretching frequencies showed that protein loses its secondary structure at temperatures higher than where we obtained crystals followed by rapid gain in β-sheet content. Interestingly, attempts to use the needles as seeds for more crystals led to "broom-like" fibril formations at the ends. Further, aggregation inhibitors like arginine and benzyl alcohol completely obliterated spherulites formation during crystallization. Refinement of crystals of lysozyme in presence of these molecules showed these small molecules bind to the interfaces of heat associated dimers and trimers. Overall our work concludes that heat induced weakly associated structures of lysozyme are the first step towards its amyloid formation.
date: 2016
volume: 6
is part of: Scientific reports
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
has document: Characterization of heat induced spherulites of lysozyme reveals new insight on amyloid initiation. (PDF)
has version: Characterization of heat induced spherulites of lysozyme reveals new insight on amyloid initiation. (PDF)
has published: Characterization of heat induced spherulites of lysozyme reveals new insight on amyloid initiation. (PDF)
subject: QR Microbiology

Characterization of heat induced spherulites of lysozyme reveals new insight on amyloid initiation. (PDF)
type: Document
has file: 82.pdf
has part: 82.pdf

NPG
type: Organization

Scientific reports
type: Collection
issn: 20452322

Neha Verma
type: Person
given name: Neha
family name: Verma

Pankaj Sharma
type: Person
given name: Pankaj
family name: Sharma

. Ashish
type: Person
given name: .
family name: Ashish

Suresh Korpole
type: Person
given name: Suresh
family name: Korpole

Pradip Kumar Singh
type: Person
given name: Pradip Kumar
family name: Singh

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primary topic: Characterization of heat induced spherulites of lysozyme reveals new insight on amyloid initiation.

QR Microbiology
type: Concept