title: Novel mutations conferring resistance to kanamycin in Mycobacterium tuberculosis clinical isolates from Northern India.
creator: Kaur, Simerpreet
creator: Rana, Vibhuti
creator: Singh, Pooja
creator: Trivedi, Garima
creator: Anand, Shashi
creator: Kaur, Amanpreet
creator: Gupta, Pawan
creator: Jain, Amita
creator: Sharma, Charu
subject: QR Microbiology
description: Twenty-nine Kanamycin resistant clinical isolates of Mycobacterium tuberculosis from Northern India were screened to evaluate genetic mutations in rrs gene, eis gene with its promoter, and whiB7 gene along with its 5'UTR. 14 strains (~48.0%) collectively exhibited mutations in rrs, eis or whiB7 target regions. While the highest frequency of mutations was found in rrs gene, eis and whiB7 loci displayed novel mutations. The novel mutations displayed by eis and whiB7 loci were found to be associated specifically with the Kanamycin resistance as none of the twenty nine Kanamycin sensitive strains harbor them. The inclusion of novel mutations of eis and whiB7 loci will be useful in improving the specificity of future diagnostics.
publisher: Elsevier Science
date: 2016-01
type: Article
type: PeerReviewed
relation: http://www.sciencedirect.com/science/article/pii/S1472979215300639
identifier:   Kaur, Simerpreet and Rana, Vibhuti and Singh, Pooja and Trivedi, Garima and Anand, Shashi and Kaur, Amanpreet and Gupta, Pawan and Jain, Amita and Sharma, Charu  (2016) Novel mutations conferring resistance to kanamycin in Mycobacterium tuberculosis clinical isolates from Northern India.  Tuberculosis (Edinburgh, Scotland), 96.  pp. 96-101.  ISSN 1873-281X     
relation: http://crdd.osdd.net/open/1916/