TY  - JOUR
N1  - Copyright of this article belongs to Elsevier Science.
ID  - open1916
UR  - http://www.sciencedirect.com/science/article/pii/S1472979215300639
A1  - Kaur, Simerpreet
A1  - Rana, Vibhuti
A1  - Singh, Pooja
A1  - Trivedi, Garima
A1  - Anand, Shashi
A1  - Kaur, Amanpreet
A1  - Gupta, Pawan
A1  - Jain, Amita
A1  - Sharma, Charu
Y1  - 2016/01//
N2  - Twenty-nine Kanamycin resistant clinical isolates of Mycobacterium tuberculosis from Northern India were screened to evaluate genetic mutations in rrs gene, eis gene with its promoter, and whiB7 gene along with its 5'UTR. 14 strains (~48.0%) collectively exhibited mutations in rrs, eis or whiB7 target regions. While the highest frequency of mutations was found in rrs gene, eis and whiB7 loci displayed novel mutations. The novel mutations displayed by eis and whiB7 loci were found to be associated specifically with the Kanamycin resistance as none of the twenty nine Kanamycin sensitive strains harbor them. The inclusion of novel mutations of eis and whiB7 loci will be useful in improving the specificity of future diagnostics.
PB  - Elsevier Science
JF  - Tuberculosis (Edinburgh, Scotland)
VL  - 96
KW  - Aminoglycosides; MDR-TB; Molecular characterization; XDR-TB
SN  - 1873-281X
TI  - Novel mutations conferring resistance to kanamycin in Mycobacterium tuberculosis clinical isolates from Northern India.
SP  - 96
EP  - 101
ER  -