creators_name: Kaur, Simerpreet
creators_name: Rana, Vibhuti
creators_name: Singh, Pooja
creators_name: Trivedi, Garima
creators_name: Anand, Shashi
creators_name: Kaur, Amanpreet
creators_name: Gupta, Pawan
creators_name: Jain, Amita
creators_name: Sharma, Charu
type: article
datestamp: 2016-10-04 05:54:48
lastmod: 2016-10-04 05:54:48
metadata_visibility: show
title: Novel mutations conferring resistance to kanamycin in Mycobacterium tuberculosis clinical isolates from Northern India.
ispublished: pub
subjects: QR
keywords: Aminoglycosides; MDR-TB; Molecular characterization; XDR-TB
note: Copyright of this article belongs to Elsevier Science.
abstract: Twenty-nine Kanamycin resistant clinical isolates of Mycobacterium tuberculosis from Northern India were screened to evaluate genetic mutations in rrs gene, eis gene with its promoter, and whiB7 gene along with its 5'UTR. 14 strains (~48.0%) collectively exhibited mutations in rrs, eis or whiB7 target regions. While the highest frequency of mutations was found in rrs gene, eis and whiB7 loci displayed novel mutations. The novel mutations displayed by eis and whiB7 loci were found to be associated specifically with the Kanamycin resistance as none of the twenty nine Kanamycin sensitive strains harbor them. The inclusion of novel mutations of eis and whiB7 loci will be useful in improving the specificity of future diagnostics.
date: 2016-01
date_type: published
publication: Tuberculosis (Edinburgh, Scotland)
volume: 96
publisher: Elsevier Science
pagerange: 96-101
refereed: TRUE
issn: 1873-281X
official_url: http://www.sciencedirect.com/science/article/pii/S1472979215300639
citation:   Kaur, Simerpreet and Rana, Vibhuti and Singh, Pooja and Trivedi, Garima and Anand, Shashi and Kaur, Amanpreet and Gupta, Pawan and Jain, Amita and Sharma, Charu  (2016) Novel mutations conferring resistance to kanamycin in Mycobacterium tuberculosis clinical isolates from Northern India.  Tuberculosis (Edinburgh, Scotland), 96.  pp. 96-101.  ISSN 1873-281X