creators_name: Gani, Zahid
creators_name: Boradia, Vishant Mahendra
creators_name: Raghu Ram, Janaki
creators_name: Suryavanshi, Prashant Mohan
creators_name: Patil, Pravinkumar
creators_name: Kumar, Santosh
creators_name: Singh, Ranvir
creators_name: Raje, Manoj
creators_name: Raje, Chaaya Iyengar
type: article
datestamp: 2018-03-27 03:19:49
lastmod: 2018-03-27 03:19:49
metadata_visibility: show
title: Purification and characterization of glyceraldehyde-3-phosphate-dehydrogenase (GAPDH) from pea seeds
ispublished: pub
subjects: QR
keywords: Characterization; Glyceraldehyde-3-phosphate dehydrogenase; NAD(+) specific; Pea seed; Purification
note: Copyright of this article belongs to Academic Press.
abstract: Glyceraldehyde-3-phosphate dehydrogenase [GAPDH, NAD + oxidoreductase (phosphorylating) 1.2.1.12] catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate coupled with the reduction of NAD(+) to NADH. In addition to its role in glycolysis, this enzyme has numerous alternate functions, in both prokaryotes and eukaryotes. In plants, additional functions have been reported from multiple species including Pisum sativum. A recent study has identified that GAPDH may play an important role in seed ageing and programmed cell death. Despite this the existing purification protocols are almost 40 years old, and only partial characterization of the enzyme has been reported. In the current study, we report a modified method for purification of enzymatically active pea seed GAPDH along with the characterization of the enzyme. Using 2D gel electrophoresis our study also demonstrates that pea seeds contain four isoforms of NAD(+) dependent GAPDH.
date: 2016
publication: Protein Expression and Purification
volume: 127
publisher: Academic Press
pagerange: 22-27
id_number: doi:10.1016/j.pep.2016.06.014
refereed: TRUE
issn: 10465928
official_url: http://dx.doi.org/10.1016/j.pep.2016.06.014
citation:   Gani, Zahid and Boradia, Vishant Mahendra and Raghu Ram, Janaki and Suryavanshi, Prashant Mohan and Patil, Pravinkumar and Kumar, Santosh and Singh, Ranvir and Raje, Manoj and Raje, Chaaya Iyengar  (2016) Purification and characterization of glyceraldehyde-3-phosphate-dehydrogenase (GAPDH) from pea seeds.  Protein Expression and Purification, 127.  pp. 22-27.  ISSN 10465928