creators_name: Garg, Roma
creators_name: Srivastava, Ritika
creators_name: Brahma, Vijaya
creators_name: Verma, Lata
creators_name: Karthikeyan, Subramanian
creators_name: Sahni, Girish
type: article
datestamp: 2018-03-26 10:24:31
lastmod: 2018-03-26 10:24:31
metadata_visibility: show
title: Biochemical and structural characterization of a novel halotolerant cellulase from soil metagenome
ispublished: pub
subjects: QR
note: Open Access
abstract: ellulase catalyzes the hydrolysis of β-1,4-linkages of cellulose to produce industrially relevant monomeric subunits. Cellulases find their applications in pulp and paper, laundry, food and feed, textile, brewing industry and in biofuel production. These industries always have great demand for cellulases that can work efficiently even in harsh conditions such as high salt, heat, and acidic environments. While, cellulases with high thermal and acidic stability are already in use, existence of a high halotolerant cellulase is still elusive. Here, we report a novel cellulase Cel5R, obtained from soil metagenome that shows high halotolerance and thermal stability. The biochemical and functional characterization of Cel5R revealed its endoglucanase activity and high halostability. In addition, the crystal structure of Cel5R determined at 2.2 Å resolution reveals a large number of acidic residues on the surface of the protein that contribute to the halophilic nature of this enzyme. Moreover, we demonstrate that the four free and non-conserved cysteine residues (C65, C90, C231 and C273) contributes to the thermal stability of Cel5R by alanine scanning experiments. Thus, the newly identified endoglucanase Cel5R is a promising candidate for various industrial applications.
date: 2016
publication: Scientific Reports
volume: 6
number: 1
publisher: Nature Publishing Group
id_number: doi:10.1038/srep39634
refereed: TRUE
issn: 2045-2322
official_url: http://dx.doi.org/10.1038/srep39634
citation:   Garg, Roma and Srivastava, Ritika and Brahma, Vijaya and Verma, Lata and Karthikeyan, Subramanian and Sahni, Girish  (2016) Biochemical and structural characterization of a novel halotolerant cellulase from soil metagenome.  Scientific Reports, 6 (1).   ISSN 2045-2322