creators_name: Kachhap, Sangita
creators_name: Priyadarshini, Pragya
creators_name: Singh, Balvinder
type: article
datestamp: 2018-03-26 10:11:38
lastmod: 2018-03-27 06:13:24
metadata_visibility: show
title: Molecular dynamics simulations show altered secondary structure of clawless in binary complex with DNA providing insights into aristaless-clawless-DNA ternary complex formation
ispublished: pub
subjects: QR
keywords: MD simulations; aristaless; clawless; minor groove width; secondary structure
note: Copyright of this article belongs Adenine Press.
abstract: Aristaless (Al) and clawless (Cll) homeodomains that are involved in leg development in Drosophila melanogaster are known to bind cooperatively to 5'-(T/C)TAATTAA(T/A)(T/A)G-3' DNA sequence, but the mechanism of their binding to DNA is unknown. Molecular dynamics (MD) studies have been carried out on binary, ternary, and reconstructed protein-DNA complexes involving Al, Cll, and DNA along with binding free energy analysis of these complexes. Analysis of MD trajectories of Cll-3A01, binary complex reveals that C-terminal end of helixIII of Cll, unwind in the absence of Al and remains so in reconstructed ternary complex, Cll-3A01-Al. In addition, this change in secondary structure of Cll does not allow it to form protein-protein interactions with Al in the ternary reconstructed complex. However, secondary structure of Cll and its interactions are maintained in other reconstructed ternary complex, Al-3A01-Cll where Cll binds to Al-3A01, binary complex to form ternary complex. These interactions as observed during MD simulations compare well with those observed in ternary crystal structure. Thus, this study highlights the role of helixIII of Cll and protein-protein interactions while proposing likely mechanism of recognition in ternary complex, Al-Cll-DNA.
date: 2017
date_type: published
publication: Journal of Biomolecular Structure and Dynamics
volume: 35
number: 6
publisher: Adenine Press
pagerange: 1153-1167
id_number: doi:10.1080/07391102.2016.1175967
refereed: TRUE
issn: 0739-1102
official_url: http://dx.doi.org/10.1080/07391102.2016.1175967
citation:   Kachhap, Sangita and Priyadarshini, Pragya and Singh, Balvinder  (2017) Molecular dynamics simulations show altered secondary structure of clawless in binary complex with DNA providing insights into aristaless-clawless-DNA ternary complex formation.  Journal of Biomolecular Structure and Dynamics, 35 (6).  pp. 1153-1167.  ISSN 0739-1102