creators_name: Kaur, Jasdeep
creators_name: Singh, Kanwar Vikas
creators_name: Schmid, Ashwini Hirlekar
creators_name: Varshney, Grish C
creators_name: Suri, C Raman
creators_name: Raje, Manoj
type: article
datestamp: 2012-01-08 05:45:26
lastmod: 2012-01-08 05:45:26
metadata_visibility: show
title: Atomic force spectroscopy-based study of antibody pesticide interactions for characterization of immunosensor surface.
ispublished: pub
subjects: QR
full_text_status: restricted
keywords: Immunobiosensor atomic force microscope; Pesticide; Affinity; Avidity adhesive force; SPR
note: Copyright of this article belongs to Elsevier science.
abstract: Development of immunobiosensor detector surfaces involves the immobilization of active antibodies on the capture surface without any significant loss of antigen binding activity. An atomic force microscope (AFM) was used to directly evaluate specific interactions between pesticides and antibodies on a biosensor surface. Oriented immobilization of antibodies against two herbicide molecules 2,4-dichlorophenoxyacetic acid (2,4-D) and atrazine, on gold, was carried out to create the active immunobiosensor surfaces. The adhesive forces between immobilized antibodies and their respective antigens were measured by force spectroscopy using hapten-carrier protein functionalized AFM cantilevers. Relative functional affinity (avidity) measurements of the antibodies carried out prior to immobilization, well correlated with subsequent AFM force measurement observations. Analysis showed that immobilization had not compromised the reactivity of the surface immobilized antibody molecules for antigen nor was there any change in their relative quality with respect to each other. The utility of the immunoreactive surface was further confirmed using a Surface Plasmon Resonance (SPR) based detection system. Our study indicates that AFM can be utilized as a convenient immunobiosensing tool for confirming the presence and also assessing the strength of antibody-hapten interactions on biosensor surfaces under development.
date: 2004-09-15
date_type: published
publication: Biosensors & bioelectronics
volume: 20
number: 2
publisher: Elsevier Science
pagerange: 284-93
refereed: TRUE
issn: 0956-5663
official_url: http://www.sciencedirect.com/science/article/pii/S0956566304000302
related_url_url: http://www.sciencedirect.com/science/article/pii/S0956566304000302
related_url_type: pub
citation:   Kaur, Jasdeep and Singh, Kanwar Vikas and Schmid, Ashwini Hirlekar and Varshney, Grish C and Suri, C Raman and Raje, Manoj  (2004) Atomic force spectroscopy-based study of antibody pesticide interactions for characterization of immunosensor surface.  Biosensors & bioelectronics, 20 (2).  pp. 284-93.  ISSN 0956-5663     
document_url: http://crdd.osdd.net/open/200/1/raje2004.pdf