TY  - JOUR
N1  - Copyright of this article belongs to Society for General Microbiology
ID  - open2001
UR  - http://dx.doi.org/10.1099/ijsem.0.002520
IS  - 1
A1  - Sudan, Sarabjeet Kour
A1  - Pal, Deepika
A1  - Bisht, Bhawana
A1  - Kumar, Narender
A1  - Chaudhry, Vasvi
A1  - Patil, Prabhu
A1  - Sahni, Girish
A1  - Mayilraj, Shanmugam
A1  - Krishnamurthi, Srinivasan
Y1  - 2018/01//
N2  - A bacterial strain, designated ASS-1T, was isolated and identified from a sediment sample of the river Ganges, Allahabad, India. The strain was Gram-stain-negative, formed straw-yellow pigmented colonies, was strictly aerobic, motile with a single polar flagellum, and positive for oxidase and catalase. The major fatty acids were C16?:?1?7c/ 16?:?1?C16?:?1?6c, C18?:?1?7c and C16?:?0. Sequence analysis based on the 16S rRNA gene revealed that strain ASS-1T showed high similarity to Pseudomonas guguanensis CC-G9AT (98.2?%), Pseudomonas alcaligenes ATCC 14909T (98.2?%), Pseudomonas oleovorans DSM 1045T (98.1?%), Pseudomonas indolxydans IPL-1T (98.1?%) and Pseudomonas toyotomiensis HT-3T (98.0?%). Analysis of its rpoB and rpoD housekeeping genes confirmed its phylogenetic affiliation and showed identities lower than 93?% with respect to the closest relatives. Phylogenetic analysis based on the 16S rRNA, rpoB, rpoD genes and the whole genome assigned it to the genus Pseudomonas. The results of digital DNA-DNA hybridization based on the genome-to-genome distance calculator and average nucleotide identity revealed low genome relatedness to its close phylogenetic neighbours (below the recommended thresholds of 70 and 95?%, respectively, for species delineation). Strain ASS-1T also differed from the related strains by some phenotypic characteristics, i.e. growth at pH 5.0 and 42?°C, starch and casein hydrolysis, and citrate utilization. Therefore, based on data obtained from phenotypic and genotypic analysis, it is evident that strain ASS-1T should be regarded as a novel species within the genus Pseudomonas, for which the name Pseudomonasfluvialis sp. nov. is proposed. The type strain is ASS-1T (=KCTC 52437T=CCM 8778T).
PB  - Society for General Microbiology
JF  -  International Journal of Systematic and Evolutionary Microbiology
VL  - 68
KW  - 16S rRNA; ANI; GGDC; P. guguanensis; Pseudomonas fluvialis; rpoB
SN  - 1466-5026
TI  - Pseudomonas fluvialis sp. nov., a novel member of the genus Pseudomonas isolated from the river Ganges, India
SP  - 402
EP  - 408
ER  -